- Imported micrographs and did template-based picking and 2D classification in cryosparc.
- Exported particles at 3D refine step (also tried at 2D step). using csparc2star.py using script below.
- Re-extracted particles in relion from original micrographs.
- All particles shifted to upper-left corner of the box, as seen by either displaying the particles or doing 2D classification.
csparc2star.py particles_selected.cs fullpath/of/outputfile/location/particles.star --passthrough passthrough_particles_selected.cs