Hi,
I am getting the following error when trying to run 2D classification after template picking:
Traceback (most recent call last):
File “cryosparc2_worker/cryosparc2_compute/run.py”, line 78, in cryosparc2_compute.run.main
File “cryosparc2_worker/cryosparc2_compute/jobs/class2D/run.py”, line 47, in cryosparc2_compute.jobs.class2D.run.run_class_2D
File “cryosparc2_compute/jobs/runcommon.py”, line 219, in load_input_group
assert is_connected, “Slot %s.%s must be connected!” % (input_group_name, slot[‘name’])
AssertionError: Slot particles.blob must be connected!
Unlike the previous user who posted about this error, I have done everything in cryosparc after importing motioncor2 mrc files. I had no problem doing CTF -> manual picking -> 2D classification/template selection -> template picking -> inspection. It is only now after the template picking/inspection do that I can not proceed in the pipeline (neither the template picking output or inspection output works). The particle stack is listed and shows in input parameters:
Not sure what to check next.
Thanks,
Christopher