Particle extractes as small set

[CryoEM2]

Selecting a subset of particles from a previous binning

Hi @Flow! If I understand correctly, you want to perform some analyses with a large pixel size to speed them up, but then return to the small pixel size for later jobs without having to reextract. This is a great workflow! Here’s my recommendation for how to do this: When extracting your particles, downsample them to a relatively small pixel size (i.e., low binning) Perform a Downsample Particles job to get your large pixel size particle stack for jobs like 3D Classification Perform jobs usin…

he’s describing “low-level inputs”, and here’s a related discussion.