Overflow Error with a Non-uniform refinement job

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#1

Hi,

I was running a Homogeneous refinement with a mask in cryosparc2 and was getting good results. However, using the same input files, when I run the Non-uniform refinement, the job crashes. This happened multiple times, giving the same error (please see the attached below). I have no clue why it’s crashing as I have used only default values. Could you kindly suggest?

Regards,
Abhinav.


#2

Hi @AbhinavKV, can you please have a look at this post - the user encountered the same error and was able to change a masking parameter to fix. Thanks!


#3

Hi Saara,

As suggested by Angel, I tried running the non-uniform refinement job again with the mask, but keeping the masking to static rather than dynamic. However, the job still crashed. I am not sure what is the exact problem. Interestingly, when I run the same job without a mask, the program runs beautifully and results in a dramatic improvement in my results as compared to the same homogeneous refinement job. But as I am sure that masking out one extremely flexible component improves my result, it is imperative to run the non-uniform refinement with the mask as it would definitely improve the results even further. Kindly find the latest error log attached with this post. Could you kindly suggest what is the right thing to do.

Regards,

Abhinav.


#4

Hi Abhinav - how did you run non uniform refinement without a mask? In my hands when I do that (e.g. setting the mask type to ‘null’) it fails with an error.

Cheers
Oli


#5

Yes, my job runs quite successfully without a mask. But i need to run it with a mask as the results will be even better i think.

Regards,
Abhinav.


#6

No, I mean how do you run it without a mask, that is, what do your inputs look like. If you just run without providing a mask, cryoSPARC will create one, and at least in my hands if I set the mask type to “null” it fails with an error.

Cheers
Oli


#7

Yes, I didn’t give a mask, but cryosparc did use its created mask in the refinement.

Regards,
Abhinav.