On 2D classification

Troubleshooting
,
#1

Hi specialists,

I am fresh to cryoSPARC, there are 2 questions about 2D classification.

  1. How can I select some classes as input for next experiment? The tab “Select 2D Classes” is unavailable(the version is V0.5.6). Only “2D classification” and “ab-initio reconstruction” are available in “New Experiment” tab.
  2. May I change the parameters of 2D classification for further run for more iterations or continue the 2D classification?

Thank you!

Regards,
Jianhao

#2

Hi @jianhao

  1. You can Select 2D Classes by first clicking on the ‘Particles’ button below the experiment result, e.g.,

image.png1166×732 146 KB

at which point the Select 2D Classes button will become active.

For more information on 2D classification in cryoSPARC, you may want to take a look at this detailed blog post.

  1. The post above discusses in depth how to change parameters to optimize 2D classification. Please let us know if this is a helpful resource.

Thanks!

#3

Hi @spunjani
Thanks!
I just viewed the experiment list in table and could find the way to choose the particles.
One more thing, if I set a 2D classification with 20 iterations. When the experiment stop, I want another 20 iteration, that are total 40 iterations. But I don’t want to start a new experiment for 40 iterations. May I continue the previous experiment of 20 iterations with more iterations?

#4

Or for some reasons, the experiment is killed and unfinished. May I restart it rather than start a new experiment?

#5