Hi Ali,
Thank you for the reply.
Structures form the refinement and sharpen look good.
The local resolution estimation failed because it can’t find the half map (I did it with Resmap).
I did the local estimation with cryosparc today and it is working fine actually. And I think I can use the result.
I attached the half map and you can have a look.
Thank you again for the help.
Best,
Wenfei