@zhenyang Running two instances requires special precautions with respect to strict separation of the instances’ databases, data paths and port ranges.
Port 39010 is not a suitable port for a second cryoSPARC instance in your case, as 39010 should be reserved for the instance running on port 39000.
You’ll also need to decide which project folders should be managed by each instance; a given project directory must not be managed by two or more instances. The guide includes a section on importing project folders to an instance.
A friendly reminder: maintain recent back-ups of your cryoSPARC database(s).
Please provide details, incl. your actions, specific error messages and screenshots, of any problems you are encountering in data migration into your new instance.