since CryoSPARC currently lacks a jobtype for focused classification (i.e. masked heterogeneous refinement) I wonder if 3D Variability analysis with a local mask could be used instead. I tried 3DVA with a local mask to classify tRNA and antibiotics in ribosome datasets. The “cluster” mode in the 3DVA-display job seems to do a decent job in sorting particles and the corresponding volumes look reasonable.
Any thoughts on this strategy?
Thanks for your answers!