I am trying to use cryosparc to run 2D classification on some negative stain micrographs. In this set I’m using, there are 22 micrographs and I picked ~20 particles per micrograph, but ran into the following error:
Traceback (most recent call last):
File “cryosparc_master/cryosparc_compute/run.py”, line 84, in cryosparc_compute.run.main
File “/home/neher-lab/cryosparc/cryosparc_master/cryosparc_compute/jobs/manual_picker/run.py”, line 643, in run_manual_picker
do_extract_particles_from_micrographs(state[‘box_size_pix’], state[‘bin_size_pix’])
File “/home/neher-lab/cryosparc/cryosparc_master/cryosparc_compute/jobs/manual_picker/run.py”, line 492, in do_extract_particles_from_micrographs
scale_const, overall_normalizer, bg_fwhm_A = 200, has_ctf=state[‘has_ctf’])
File “/home/neher-lab/cryosparc/cryosparc_master/cryosparc_compute/jobs/extract/extraction.py”, line 90, in do_extract_particles_single_mic
mic -= bg_full # bg subtracted
ValueError: operands could not be broadcast together with shapes (4090,4081) (4088,4072) (4090,4081)
I’m running cryosparc v3.2.0.
Thanks for any insights.