Manual Picker: Value Error

I am trying to use cryosparc to run 2D classification on some negative stain micrographs. In this set I’m using, there are 22 micrographs and I picked ~20 particles per micrograph, but ran into the following error:

Traceback (most recent call last):
File “cryosparc_master/cryosparc_compute/”, line 84, in
File “/home/neher-lab/cryosparc/cryosparc_master/cryosparc_compute/jobs/manual_picker/”, line 643, in run_manual_picker
do_extract_particles_from_micrographs(state[‘box_size_pix’], state[‘bin_size_pix’])
File “/home/neher-lab/cryosparc/cryosparc_master/cryosparc_compute/jobs/manual_picker/”, line 492, in do_extract_particles_from_micrographs
scale_const, overall_normalizer, bg_fwhm_A = 200, has_ctf=state[‘has_ctf’])
File “/home/neher-lab/cryosparc/cryosparc_master/cryosparc_compute/jobs/extract/”, line 90, in do_extract_particles_single_mic
mic -= bg_full # bg subtracted
ValueError: operands could not be broadcast together with shapes (4090,4081) (4088,4072) (4090,4081)

I’m running cryosparc v3.2.0.

Thanks for any insights.

Hi @akw, can you please let us know which program you used for motion correction?