Local resolution range

Hi @huqi,

Just to clarify - are you looking for the best way to visualize the local resolution of a protein structure that you have passed through a Local Resolution Estimation job? The easiest way to visualize the actual local resolution values would be to use UCSF Chimera.

Specifically, you can

  1. Download the sharpened map from whichever refinement you are considering, as well as the map_locres volume output from the Local Resolution Estimation job.
  2. Open both volumes in UCSF Chimera
  3. Select the volume corresponding to the actual sharpened map, and then click Tools > Surface Color
  4. Change the settings to color the sharpened map by “volume data value”, and for the volume file, select the map_locres mrc file that you downloaded.
  5. Click “Color”. You can also manually change the values assigned to each color sample

Here’s an example of the Surface Color dialog box next to the map, colored by resolution:

Let me know if this answers your question, or if you need any clarification! You can also find more information in this post: View coloured 3D map in Chimera using output from local resolution

Best,
Michael

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