I am attempting to run local resolution estimation in v2.13.2 using the 0.143 FSC threshold and an adaptive window factor of 35 (based on the recommendations from the GUI mouse-over text). All other settings are default. Using GPU for calculations results in a CUDA memory allocation error (GTX 1080Ti, 256 box size), so I am trying CPU instead. However, the percent of voxels completed started at over 100% when I began the job and after letting it go over night , it is still running and says it is 760% complete.
I did run my non-uniform refinement with the default settings of FSC >= 0.5 and AWF = 6.
Running Local Resolution using the 0.5 FSC, 6 AWF and GPU compute settings seems to work just fine and gives a good map. It would be nice to know what it would look like with the other settings however. I’ll leave this running a bit longer and see if it finishes.
Is this still an issue? were you able to run the job to completion?
It did in fact go to completion (in 203288 seconds).
I am curious still about the AWF recommendations however. I have not been able to use the recommended AWF of greater than 35 in NU refinement or local resolution estimation with GPU. It always errors with a CUDA memory allocation error (we have 4x GTX-1080 Ti). I think the highest I have been able to successfully use is 12.
Edit: Just saw your other post about using an AWF as low as 3, which runs a lot better for me. If you hover the mouse cursor over the AWF entry field it suggests to use >35 for FSC>=0.143 . This gives an essentially flat graph for local resolution across the whole particle. Does that seem right?
Ah, the value of 35 in the tooltip is a typo, it should be 3, we will fix this! The value as low as 3 is recommended as you saw in the other post.