Local Motion with .eer

Troubleshooting
1

i am trying to run a local motion correction (per particle) with an input of eer. but i get the following error. Thoughts?

  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/cryosparc_compute/jobs/runcommon.py", line 1726, in run_with_except_hook
    run_old(*args, **kw)
  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/deps/anaconda/envs/cryosparc_worker_env/lib/python3.7/threading.py", line 870, in run
    self._target(*self._args, **self._kwargs)
  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/cryosparc_compute/jobs/pipeline.py", line 86, in stage_target
    work = processor.exec(item)
  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/cryosparc_compute/jobs/pipeline.py", line 43, in exec
    return self.process(item)
  File "cryosparc_worker/cryosparc_compute/jobs/motioncorrection/run_local.py", line 133, in cryosparc_compute.jobs.motioncorrection.run_local.run_local_motion_correction_multi.motionworker.process
  File "cryosparc_worker/cryosparc_compute/jobs/motioncorrection/run_local.py", line 173, in cryosparc_compute.jobs.motioncorrection.run_local.run_local_motion_correction_multi.motionworker.process
  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/cryosparc_compute/blobio/mrc.py", line 137, in read_mrc
    data = read_mrc_data(file_obj, header, start_page, end_page, out)
  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/cryosparc_compute/blobio/mrc.py", line 88, in read_mrc_data
    dtype = mrc_datatype_to_dtype(datatype)
  File "/home/bio21em1/Software/cryosparc3/cryosparc_worker/cryosparc_compute/blobio/mrc.py", line 67, in mrc_datatype_to_dtype
    assert False,'Unsupported MRC datatype: {0}'.format(datatype)
AssertionError: Unsupported MRC datatype: 650709282
2

Hi @ehanssen,
Local motion correction should support .eer files - did you already import the .eer data and run it successfully through patch-motion?

3

Yes the patch motion in cryosparclive seems to have run just fine. But maybe I should expand. Since the job ran as cryosparclive I had to export the chosen exposure before running the per particle motion correction. I tried re-extracting the particle from the exported exposure but this gives the same error

4

Any news on this front?

5

Hi @ehanssen we have found a bug and will be releasing a patch with the fix next week!