Local decomposition in NU local refinement

[zhangrui_wustl]

Hi, @apunjani, thank you so much for the explanation!
Please see my other post below. For some reason I consistently got ~1Å worse resolution using the new local refinement compared to the legacy one. And overall the new job run even slower than the legacy one And suggestions would be greatly appreciated!

Search range in Local Refinement (Legacy)

Dear Michael, Thank you so much for the explanation! I’ve also tried the Local Refine (New beta) with “Use pose/shift gaussian prior during alignment” on. The plot looks much better (see below). However, the achieved resolution is significantly lower. For example, I can get 4.1 Å with the legacy one, but 5.0 Å for the new one. [Screen Shot 2021-05-11 at 2.39.56 PM] Any suggestion to tweak the parameters would be greatly appreciated! The parameters I used for the new job is like this: [Sc…