We’ve been feeling a somewhat opposite problem: We find that Live 2D classification reveals many more alternative views of the particle - I’m reluctant to call them rare views because they can be quite populous in Live distributions, but seem rare and get hidden in the non-Live 2D classification distributions.
It seems concerning that the algorithms yield different results, since good reconstructions depend on more views, and users might miss these by not running Live for many datasets. For what it’s worth, we see a similar phenomenon now that RELION 4 uses an algorithm that 2D classifies faster and puts out more “fuzzy blobs” and hides rare views (fast subsets in RELION 3 had a similar but not as bad effect). The field seems to be going in the direction of fast and dirty 2D classification, and maybe Live gives a more slow and careful sorting, unintentionally? Any advice about workarounds that bring the normal and Live methods in sync and make sure normal mode doesn’t hide views?