Hi, After initial processing in Cryosparc Live, I am performing some manual processing in normal Cryosparc. Strangely, my 2D classes worked much better in Live than in normal CS, even using the same particle stack as input. I’ve tried to copy the conditions for the Live 2D classification (number of…

Thanks for the suggestions and for linking this in with the existing discussion. I found that re-extracting the particles from Live using refined shifts gave me satisfactory results in subsequent 2D.