Launching Dataset Error: Float Division by Zero


My Cryo-EM data set was collected with a tilt of 40 degrees. I performed a local Gctf in order to correct the values for the tilt. I made sure that all of the value changes were correct. However when I try to launch the data set for the corrected Gctf, this is the error I get.

Launched job jFsT9bdPQmZeszeyr
License is valid.
Job Type: visualize_dataset Job ID: jFsT9bdPQmZeszeyr
Assigned Slots: RAM: None GPU: None
Visualizing Dataset ID BJKMBXYawy8EeiCEr
Loading Dataset …
---- Loading File Set. Type: star Path: /share/apps/cryosparc/run/bulk/./correct_f3/
Traceback (most recent call last):
File “/share/apps/cryosparc/cryosparc-compute/”, line 115, in
dataset = dataio.dataset_factory(current_dataset_params, do_cache=False, do_datasign_estimation=False)
File “/share/apps/cryosparc/cryosparc-compute/dataio/”, line 18, in dataset_factory
File “/share/apps/cryosparc/cryosparc-compute/dataio/”, line 57, in filesets_into_dataset
images = extension_map[meta_type](dataset, meta_input)
File “/share/apps/cryosparc/cryosparc-compute/dataio/”, line 321, in star_into_dataset
p.psize = 10000 * p.detector_psize / p.mag
ZeroDivisionError: float division by zero

But when I check for the values that is giving the supposed error, as shown below, none of them are zero, as surrounded in red in the image below. One of the previous discussions regarding a similar issue mentioned the problem as a bug. I would like to know if there is anything I can do to alleviate the issue so I can launch this corrected data set into cryoSPARC. Please and thank you.


I was able to upload a with local CTF estimates by extracting the particles through Relion2.1 using the as the coordinate files and a that only contained the micrographs names and manually setting the pixel size for the extraction. This set of steps might be a work-around.

Hi @Cper13, sorry for the delayed response - would it be possible for you to zip the problematic star file and email to redacted@email_address ? That way we can reproduce and debug the problem.