Inconsistent definition of GS-FSC based resolution limits in homogeneous and local refinement [Bug]

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(Olibclarke) #1

Hi,

The resolution estimation bug discussed some time ago (Resolution in FSC), where cryoSPARC estimates the resolution as the highest resolution where the FSC crosses 0.143, rather than the lowest, is no longer present in Homogeneous refinement jobs, but it still persists in local refinement (I haven’t checked non uniform). See attached screenshots - one with local refinement, the other with homogeneous.

Would be good to have a fix, and also to use the corrected FSC during intermediate iterations of local refinement - presently, if small masks are used, local refinement has a tendency to spiral towards unrealistically high resolutions. Using the corrected FSC to set res limits for alignment at intermediate iterations might help with this.

Cheers
Oli