hi again, I ended up getting the coordinates imported, then realized that my original picks were from 2x binned images (collected in super-res), while the image alignment in cryosparc apparently cannot do binning? So my idea to fix it was simply edit my input particles star file so that the X and Y coordinates are twice their value (using awk and simple 2*$1). Now the coordinates make sense on an unbinned super-res image. However when I try importing as described above (with everything working fine before) I get the error below. Actually I guess I also need to multiply the relion origin x/y. Hmm.
anyways, here’s the error:
Attributeerror: ‘float’ object has no attribute ‘rFind’.
Example of a particle before and after my modification (where columns 1 & 2 are X/Y coords). Main difference is the number of decimals.
before:
1688.778408 1939.250000 0.070000 101.200316 1 0.121375 19.670000 10938.519531 11022.889648 1.050000 032511@stacks/fromrelion.mrcs 10000.000000 image01.mrc 0.000000 2.700000 300.000000 1961 139.397835 89.142762 0.501717 4.281999e+05 0.442032 2 -0.44262 -4.52012 0.000000 1.000000 3.302000 2
after:
3377.56 3878.5 0.070000 101.200316 1 0.121375 19.670000 10938.519531 11022.889648 1.050000 032511@stacks/fromrelion.mrcs 10000.000000 image01.mrc 0.000000 2.700000 300.000000 1961 139.397835 89.142762 0.501717 4.281999e+05 0.442032 2 -0.44262 -4.52012 0.000000 1.000000 3.302000 2