Hi you all,
To sum up, I performed Motion correction, CTF estimation in CryoSparc and micrographs seleccion in CryoSparc. Then, I selected manualy the start-end coordenantes of my filaments in Eman2 and save the coordenates.
Before coming back to cryoSparc, I imported those coordenates into Relion and extract them so I would obtain the right .star file to import again the particles into CryoSparc.
The problem comes when I extract the particles in cryosparc linking both imported particles and curate micrographs, the CTF values are not taken.
Would you have any idea of how to solve this?
Thanks in advance
Sergio BARATA