How do I reconstruct my whole protein after local refinement

[Adria_AJ]

Hello,

I just ran two local refinement in paralel, one for each masked region of the protein and now I am wondering how to reconstruct the full map again and not having two. Is there a way to do it in Cryosparc?

Thank you very much!

A.

[wtempel]

@olibclarke

For map combination, aligning maps to a global reconstruction and combining by taking the maximum value at each voxel (vop maximum in UCSF Chimera) is the best method I have found to get a seamless combination. I would still encourage deposition of the orginal component maps as well, though.

Would this suggestion from a related forum topic work for you?

[ajm]

The previous suggestion of using vop maximum worked fine for me, and I was able to create a combined map for model building, though I can’t run a local resolution estimation job with this new map, as after importing the volume, there are no half maps. Is there any way to get a local resolution map made of this new combined map?