How to combine two local-refined maps of two parts from one protein into one map in Cryosparc?

Hi,

In the “new” local refinement in 3.1, each part remains in its original location - not recentered in the middle of the box. This makes map combination and other downstream operations such as particle subtraction more convenient.

For map combination, aligning maps to a global reconstruction and combining by taking the maximum value at each voxel (vop maximum in UCSF Chimera) is the best method I have found to get a seamless combination. I would still encourage deposition of the orginal component maps as well, though.

Cheers
Oli

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