Homogenious refinement of full particles after 3D classification with particle subtraction

[nbustorff]

Hi,

I am trying to do 3DVA and 3D classification with particle subtraction and mask and would like to refine the full particles after classification? Is it possible to do it in cryosparc?

I don’t know if it would be something similar with the downsample like in this thread:

Unbinning particles using 'Downsample particles' job in CRYOSPARC

Hi I have imported particles from RELION and binned them 2x in CRYOSPARC using the ‘Downsample particles’. Later I have done a classification scheme in CRYOSPARC on the binned particles. I have NOT imported the motion-corrected-micrographs from RELION. Is it possible to use ‘Downsample particles’ to unbin the particles after classifying them in CRYOSPARC? I am using CRYOSPARC v2.5.0. Thanks Quereshi

Best regards,
Nuno

[olibclarke]

Hi Nuno, yes, you can do it exactly as described in the linked thread. Just replace the particles low level input slot (which corresponds to the signal subtracted particle images) with the one from the originally extracted particles (before classification).

Cheers
Oli

[nbustorff]

Hi Oli,

Thank you very much for the quick reply.

Best regards,
Nuno