Homogenious refinement of full particles after 3D classification with particle subtraction


I am trying to do 3DVA and 3D classification with particle subtraction and mask and would like to refine the full particles after classification? Is it possible to do it in cryosparc?

I don’t know if it would be something similar with the downsample like in this thread:

Best regards,

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Hi Nuno, yes, you can do it exactly as described in the linked thread. Just replace the particles low level input slot (which corresponds to the signal subtracted particle images) with the one from the originally extracted particles (before classification).


Hi Oli,

Thank you very much for the quick reply.

Best regards,