I’m new in cryo-EM data processing and I’m trying to do the heterogeneous refinement, but I got this error message:
Traceback (most recent call last): File "cryosparc2_worker/cryosparc2_compute/run.py", line 82, in cryosparc2_compute.run.main File "cryosparc2_worker/cryosparc2_compute/jobs/hetero_refine/run.py", line 476, in cryosparc2_compute.jobs.hetero_refine.run.run_hetero_refine TypeError: 'NoneType' object is not subscriptable
Does anyone know what this means?
What I did for now:
- Rounds of 2D classification (remove some junks)
- Ab-initio reconstruction with 3 classes
- Heterogeneous refinement. My inputs for heterogeneous refinement are: the 3 volumes from ab-initio reconstruction and all particles from ab-initio reconstruction.
Am I missing something?