Help with merging particles from 2 datasets

Troubleshooting
3

Hi @mmclean,

Thanks for getting back to me so quickly.
Yeah, we have collected them 3 months apart at the same microscope. That means that our dataset A was processed in an older version of the CryoSPARC, I doubt that this is a problem, right? The only difference between the datasets was the spot size…

Now, I’ve turned on Min over scale after first iteration in 2D Classification but there is an error message:

2D_Min_over_error
2D_Min_over_error1886×537 308 KB

2D_Min_over_class
2D_Min_over_class4488×2248 1.1 MB

I tried to turn it on for datasets A and B individually and the same problem occurred.

Any thoughts?
Best,
Gio