Export 3DVA results in chimera as movie

3D Variability Analysis
1

Hi, all!
I met a problem after doing the 3DVA.
I used the “simple” mode in the 3DVA. And I opened the 20 frames in Chimera via “volume series”. Now it can be a video in the Chimera. However, I wanted to export the video as .mp4 or other file type. How can I realize it? I am so sorry maybe it is a question about Chimera. But I believed that maybe someone here can give me a suggestion. Thanks a lot!

2

Chimera question, LOTS of useful resources on google and YouTube for chimera or chimeraX about Movie Maker. Quick tip if you’re on Mac, command + shift + 5 should record movie of any portion of the screen for any amount of time. Just play series and record

3

Hi @Yuqi I often use the following method with chimera.

Step1 : Volume Series setting

  • Play direction: oscillate
  • Check ‘Normalize threshold levels’
  • Check ‘Cache 30 renderings’
  • Data cache size : 1024 Mb

Step2 (optional) : Disable auto adjusting of step upon opening maps

  • Volume Viewer > Features > Data display options
  • Uncheck “Adjust step to show at most …”

Step3 (optional) : Always open maps with the default grey color

  • Volume Viewer > Features > Data display options
  • Uncheck “Initial colors”

Step4 (optional) : Set appropriate windows size

  • Specify width x height (in pixels) of movie with windowsize command.
  • For example: windowsize 480 480

Step5 : Create movie

  • Try this command. (Assuming the volume series is model #0)

vol all hide; movie record; vop morph #0 playStep 0.05 frames 20; wait 20; movie encode ~/test.mp4 framerate 20

To generate a movie of N round-trips of the volume series, replace the argument of ‘frames’ and ‘wait’ by 40 * N. For example to make a 2 round-trip movie,

vol all hide; movie record; vop morph #0 playStep 0.05 frames 80; wait 80; movie encode ~/test.mp4 framerate 20

(‘framerate’ should be left at 20)

Hope this helps

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