I am following along the steps in the T20S Tutorial with my own data set and have encountered an issue when attempting to carry out local motion correction following particle picking. The error encountered is below:
Traceback (most recent call last):
File “cryosparc2_worker/cryosparc2_compute/run.py”, line 49, in cryosparc2_compute.run.main
File “cryosparc2_compute/jobs/runcommon.py”, line 325, in check_default_inputs
assert False, 'Non-optional inputs from the following input groups and their slots are not connected: ’ + missing_inputs + ‘. Please connect all required inputs.’
AssertionError: Non-optional inputs from the following input groups and their slots are not connected: movies.background_blob. Please connect all required inputs.
If I understand correctly, it’s looking for a file that should be connected to this job called movies.background_blob, but in looking through the outputs from importing the movies up to this step, I can’t seem to see any files that have that name.
Has anybody else encountered this issue? I am very much a beginner with cryo-EM data processing and it’s entirely possible that I’m missing something obvious.