Hi,
I am new to EM and have been trying to move my extracted particles from a refinement job from CryoSPARC to relion and running a 3D initial reference job, It starts fine and runs till iteration 89 and then gives me this error:
psi_angles[ipsi]= 0 prior_psi= 45
diffpsi= 45 sigma_cutoff= 3 sigma_psi= inf
in: /software/relion/5.0/relion/src/healpix_sampling.cpp, line 979
ERROR:
prior on psi is zero!
=== Backtrace ===
/software/relion/5.0/bin/relion_refine(_ZN11RelionErrorC1ERKNSt7__cxx1112basic_stringIcSt11char_traitsIcESaIcEEES7_l+0x7d) [0x55e1f26ca86d]
/software/relion/5.0/bin/relion_refine(+0x8782c) [0x55e1f268882c]
/software/relion/5.0/bin/relion_refine(_ZN11MlOptimiser27expectationSetupCheckMemoryEi+0xc4b) [0x55e1f26eebcb]
/software/relion/5.0/bin/relion_refine(_ZN11MlOptimiser11expectationEv+0x154) [0x55e1f2731cc4]
/software/relion/5.0/bin/relion_refine(_ZN11MlOptimiser7iterateEv+0xdb) [0x55e1f273cceb]
/software/relion/5.0/bin/relion_refine(main+0x6f) [0x55e1f26b89ef]
/lib/x86_64-linux-gnu/libc.so.6(__libc_start_main+0xf3) [0x7fe3d8b4b083]
/software/relion/5.0/bin/relion_refine(_start+0x2e) [0x55e1f26bc65e]
ERROR:
prior on psi is zero!
Thanks for your help!