when the ab-initio gives one class that looks symmetric, lets say C6, I know that in the following refinment we can enforce this C6 symmetry and basically increase the number of particles by doing so (each image of certain views will contribute to all 6 symmetry views).
I would liek to know, is what we are getting the actual structure? I always thought that enforcing anything is contraproductive and can give results that look nice, but are not true. With C6 set, will we get a strucuture with 6 identical subunits? Or might they still have slight differences. In other words, is CS calculating only one subunit and simply multiplying it 6 times in the correct arrangement?
What result would we get if we leave C1?
I am referring to the beginning of the tutorial: https://www.youtube.com/watch?v=HXIipHORI2Y
(extremely helpful sessions by the way!)