Corrected Mask issue in Non uniform refinement GSFSC

3D Refinement
1

I am running Non uniform refinement with the default settings, 100K particles (output of heterogeneous refinement job), a volume input (~7 Angstrom output of a Heterogeneous refinement job), and no static mask input. The job runs, but then finishes after 3 iterations, and the corrected mask in the FSC plunges down vertically to 0 at around 6-7 Angstroms. The output is a low quality map, when I would expect something closer to the 3 Angstrom range. This happens regardless of what volume input I use. Any thoughts? Is this an issue with the dynamic masking? I have never needed to input a static mask for this job in the past. Im happy to provide additional information to clarify my situation if it would help! Here are the GSFSC outputs

Screen Shot 2022-12-27 at 1.01.38 PM
Screen Shot 2022-12-27 at 1.01.38 PM1716×1430 222 KB

Thank you

2

Welcome to the forum @czerio .

Did you confirm that this particular dataset can in fact produce a 3 Å resolution model? Has the gain reference been correctly applied during movie import?