Hi,
I want to do some “local search” of rotation angle after some minor “picking” of the particles, and the corresponding job in RELION is “Continue from here” in 3D refinement. I wonder if there is a similar job in cryoSPARC where I can give a initial value to the rotation angle and make them change just a little bit.
Local refinement job is meant to mask a part of the volume and make the small part more accurate, but I just want to do a “local search” of rotation angle using the whole volume. Also I tried Local refinement job after homogenous refinement, and found the resolution decreased a little instead.
Any advice would help a lot!