I have two questions regarding 3D variability analysis.
- After doing 3DVA in cluster mode I took the particles corresponding to the best-looking cluster and run NU refinement. The resulting map looks worst than the map I got from the 3DVA. Why is that happening if both have the same particles? How is refinement in the 3DVA job different from NU refinement?
- Since the 3DVA cluster map is looking better than any other map I have, could I use the 3DVA map for publication? How can I get half maps of it? and estimate resolution?
Thanks for your insights!