Can I use Non-uniform refinement for 3D variability?

3D Variability Analysis
1

Hi,

I am wondering if I can use the Non-uniform refinement result as the input of 3D variability. The tutorials or the related posts I read only seem to mention homogeneous refinement results as the input of 3D variability, so I’m not sure if it is ok to use NU refinement results instead. Would the maps yielded by NU and homo refinement lead to different 3D variability results?

The protein I work on is about 100kDa and is quite flexible. NU refinement yield better maps than homogeneous refinement. So I want to use the NU refinement map as the input for 3D variability analysis if that is also appropriate.

I’d appreciate any advice. Thank you.

Best,
Meiling

2

Yes, you can use results of NU-refine for 3D-VA.

3

Thank you so much @olibclarke for the confirmation!

Best,
Meiling