Calculate FSC in cryosparc using external half maps

[smallelephant9516]

How could we use the half maps generated in other softwares (for example, relion) to calculate the FSC in cryosparc using the validation tools. It seems that the validation tools can only import 1 half map. This indicate that one need to link two half maps together. It seems that we can import the half A and B individually but how could we link these two together. Thanks for any advices!

[T_Bird]

Can't input two half maps into Validation (FSC) as described in "Local res. estimate with imported map error"

Hi @zorg, Apologies for the very delayed reply. Once you have dragged the map_half_A group (from the first volume) into the volume slot, you have to drag the map_half_B low-level result (from the second volume) into its result slot. When you click and drag the map_half_B result into its slot in the volume input, you should see both slots turn green. You can then drop it on one of the slots, and it will override the duplicated map_half_A result. For example, see the screenshot below of what it s…

Tutorial: Job Builder | CryoSPARC Guide

[smallelephant9516]

Thanks, it really solve the problem