C2 symmetry with symmetry breaking features

Hi Bassem, the map looks very promising and like you are on the right track. See the discussion here for some recommendations on how to assess pseudosymmetry in cryosparc.

At first glance, I would try symmetry expansion, followed by local refinement with a mask on the peripheral domain (or single protomer), followed by 3D classification without alignments/shifts in relion using a similar peripheral domain mask. Does 3DVA demonstrate large motions on the periphery and inform on where to place a mask and fulcrum for local refinement?