Hi,
If beam tilt parameters are included in an imported star file (rlnBeamTiltX/Y), are they used in the refinement at all?
CryoSPARC 2 seems to read the beam tilt columns (obtained using CtfRefine in Relion 3) from the star file when importing, but I don’t know whether it is incorporating correction for the beam tilt based on these parameters or not.
I suspect not, as the resolution doesn’t seem to change at all, but I just want to make sure.
Cheers
Oli