I have been combining particles from different datasets without problems.
I am now trying to re-process and repeat this but keep getting this error when trying to run a refinement with the combined particles from different datasets:
Traceback (most recent call last): File "cryosparc_worker/cryosparc_compute/run.py", line 84, in cryosparc_compute.run.main File "cryosparc_worker/cryosparc_compute/jobs/hetero_refine/run.py", line 63, in cryosparc_compute.jobs.hetero_refine.run.run_hetero_refine File "/xxx/software/cryosparc/cryosparc_worker/cryosparc_compute/particles.py", line 39, in init assert n.all(n.abs(self.data['blob/psize_A'] - self.psize_input) < 1e-4 ), "All particles must have the same pixel size to within 10^-4 Angstroms" AssertionError: All particles must have the same pixel size to within 10^-4 Angstroms
I have been able to do this before without problems, and the datasets have exactly the same pixel size, so I don’t know what is happening and why now I cannot process it again…
Any help would be greatly appreciated!