My cryoSPARC version is 0.6.1
I tried to run heterogenous refinement with icosahedral symmetry on icosahedral virus data. I tried to set option “Symmetry” with I or I1, but It didn’t work. The error output is following.
CAUTION: RUNNING EXPERIMENTAL MULTIREFERENCE REFINEMENT ====== Multireference Refinement ====== Refining Structures with volume size 256. Traceback (most recent call last):
File “/home/lthpc/Documents/jianhaoc/cs/cryosparc-compute/sparcjob.py”, line 562, in
cuda_devs = current_experiment_params[‘cuda_devs’])
File “/home/lthpc/Documents/jianhaoc/cs/cryosparc-compute/multirefinement.py”, line 65, in multirefine
assert params.refine_symmetry == ‘C1’, “Symmetry is not yet supported in multireference refinement”
AssertionError: Symmetry is not yet supported in multireference refinement
While I try C1 symmetry, it works. However, the reconstructed map doesn’t show a icosahedral solid.