AssertionError during CTF Refinement job: Says ctf/cs_mm is not constant and that it should be a value I never inputted

Hi,

While runing a Global CTF Refinement (BETA) job with outputs coming from a Non-uniform Refinement (NEW!) I ran into this AssertionError. It says my cs_mm should be 3.1690547 but I never inputted that value, I actually put 2.7 mm.

I had run this job before and it went throiugh with no problems, but then I re-extracted my particles with a bigger box size, ran them through Non-uniform Refinement and then into this job and now it doesn’t work.

I’ve tried messing with the parameters but alas, it still fails.

Does somebody know a fix for this?

Best,
Armando

Hi,

Probably you have refinement of global CTF parameters selected in non uniform? That would explain why it is 3.17, but not why it is not constant - are you inputting particles from multiple jobs?

Cheers
Oli

Hi Oli,

I did.

However, I just tried doing the same job, with the same patameters, with the outputs of a single Homogenous refinement job that had particles straight from an extraction job and it gave the same error.

Thanks,
Armando