Anyway to directly measure the refinement modle length?

is there any tool in cryosparc or chimerax that can tell me the longest and shortest length of refinement modle?
thanks a lot!

Hi,

Assuming a volume, perhaps a rough approximation can be derived using measure inertia in ChimeraX. Alternatively, you can manually place surface markers at what are judged to be the extreme ends and measure the distance between them. Both methods require user-judgement for appropriate contour level.

If the volume was generated ab initio in cryoSPARC initially, the longest meaningful dimension is arbitrarily aligned along the z-axis.

If handling an atomic coordinate model, something like this Draw Protein Dimensions script in PyMOL could be handy. However, this is limited to the space occupied by atoms.

Cheers,
Yang

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appreciate a lot! :heart: :heart: :heart:

I wasn’t aware of measure inertia - very neat! I had always used the marker approach, but that is much cleaner & faster, thanks!

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