I did a local refinement job, there was a dip in the reported corrected FSC after auto-tightening. Does this look normal？ and what reason lead to this？
Also, I have a question about results in corresponding directory. There were several mrc files, which map is the corresponding map to the corrected FSC?
Please see this thread for a previous discussion/answer to your first question about the FSC curve.
For your second question, the corrected FSC is calculated by applying the
..._mask_fsc_auto.mrc mask to the two raw half-maps (located in the
As well, the
volume_map.mrc is filtered by the “tight mask” FSC curve. The
volume_map_sharp.mrc is the same as
volume_map.mrc, but with one extra step of being sharpened to the estimated B-factor.