3DVA using the mask called mask_fsc_auto

3D Variability Analysis
#1

Hi all,
I found that the mask(the output of Nonuniform refinement）called cryosparc_volume_mask_fsc_auto.mrc only included the protein parts in my memprane protein. I was wondering whether I could use this type of mask for the following 3DVA protocol. Any bad influences?

Thank you very much!
Peter

#2

If the mask looks sane and covers the parts you’re interested in, it will be fine. IIRC that mask can have some values close to, but less than, 1 inside the mask, so you might want to check that and possibly re-threshold it.

#3

I got it. Thank you so much! :smiley: