3D refinement working well in older version but doesn't work in version v4

3D Refinement
1

Hi,

I met an issue when running the new version cryoSPARC v4. The same cluster of particles can be reached to the resolution of 3.2Å after running the non-uniform refinement in previous version cryoSPARC before, however, it’s strange that the iteration stopped very ealier (3-4 iterations, in contrast to 13-14 iterations before) and resolution I get is 6.5 Å when I run these same particles in the nonuniform refinement in cryoSPARC v4. Same problem happened in another dataset that can be resolved quite good by running non-uniform refinement in the older version. Very appreciated for any explanation and suggestions.

2

Welcome to the forum @Peng.
Do you still have available an “original” NU-refinement job:

  • that ran on the mentioned previous version of CryoSPARC and reached 3.2 Å resolution.
  • whose upstream inputs have not been overwritten (for example by clearing and re-running upstream jobs that provided input for the original NU-refinement)

?
I you do, please can you

  1. clone the “original” job
  2. run the cloned job on CryoSPARC v4
  3. email us the job reports of the “original” and cloned jobs.

Thanks.