3D classification gives similar percentage of particles of each class

[cbeck]

Hi Wendy!

In my experience, just turning on “Force Hard Classification” really helps with preventing particles from being equally distributed across all classes. You can find some further information on what parameters to play with in this thread:

3D Classification strategy: Classify until exhaustive?

Hi everyone! Today I would like to ask a question on your strategy involving the use of 3D Classification for peeling may conformations from a dataset of a wildly active protein. I have been using 3D Classification on 800k particles (selected by many rounds of 2D classification to remove junk particles) and split into 4 classes consisting of 180k, 250k, 198k, 203k particles each. Then, I realize that each class can be split into 2-3 more classes. For one of those subclasses consisting of 40k p…

Cheers,
cbeck