Hi all,
I am seeking advice regarding B-factors in a PDB file. I have completed model building, but when I open the PDB file, I notice that many atoms have either zero or unusually low B-factors which I believe should not be the case. I tried running real-space refinement in phenix after replacing zero B-factors with random values, but it didn’t work. I would appreciate any insights or suggestions on how to address this.
Thanks in advance