Hi
I tested 10 imported motion corrected (MotionCor2) micrographs by CTFFIND4 in CS. The thon rings and CTF plot looks alright. The intensity column is still zero.
I tested the same 10 impoted micrographs by patch CTF estimation in CS. The thon rings look very odd.
I also tested 10 original movies: MotionCor2 then patch CTF, the thon rings look very odd.
I checked the T20S tutorial dataset, patch motion corretion plus patch CTF estimation, had intensity numbers.
So I wonder patch CTF would need patch motion correction micrograghs as input?
(Our hardware can’t run patch motion correction. So I am trying to walk around to generate or import pre-processed micrographs to CS).
Many thanks for your help.
Kind regards,
Yahui