Zero dose extrapolation

Features and Functionality
1

Hi CryoSPARC community, I haven’t used CryoSPARC in a while, just wondering if zero-dose extrapolation is now available in the latest release?

2

Hi @Mayin,

Thanks for your inquiry! Currently, we do not offer an option for 0-dose extrapolation during reconstruction. It is worth noting however that radiation damage is accounted for during dose weighting through use of a standard dose weight curve (in e.g., Patch Motion) or an empirically derived dose weight curve if Reference-based Motion Correction is performed.

We have a few follow up questions regarding your use case below:

  1. Do you currently have a use case where you believe 0-dose extrapolated reconstructions would be beneficial?

  2. Are you already using HexAuFoil grids for your projects?

Best,
Kye