Hi @hemomona,
I am wondering if this is a similarly related issue to a previous post? What processing steps did you do previous to this TOPAZ train job? Its best to ensure that the particles you are using are from the micrographs you connect to the job and that the micrographs in the TOPAZ preprocess directory are not from a previous TOPAZ train/extract job where the binning of the micrograph has changed.
Best,
Kye