Looking at the error message: "Invalid path specified:...",
I’d check the tiff files path again. Make sure you are including the tiff files with wildcard characters after the movies directory path e.g. /srv/data3/cryosparc_user/CryoEM_DATA/Test_EMPIAR11012/*tif
Thank you so much! I did not realize that we have to manually type the wildcard characters! I will try this out. I had another question, when my jobs fail, is there a way to retrieve information about why it failed?