What is .mrc file?

Import and Export
1

Hi everyone,
I am new to cryosparc. while importing the .tif files and .mrc file, I got the following error.

[CPU: 359.8 MB] Traceback (most recent call last):
File “cryosparc_master/cryosparc_compute/run.py”, line 85, in cryosparc_compute.run.main
File “/efshome/cryosparcuser/v3.3.2_220824/cryosparc_master/cryosparc_compute/jobs/imports/run.py”, line 1044, in run_import_movies_or_micrographs
imgdata = tiff.read_tiff(abs_path).sum(axis=0) * gainref
ValueError: operands could not be broadcast together with shapes (7676,7420) (3710,3838).

Based on the error, I understand the tiff arrays and mrc arrays are not equal. And, also I would like to know what is the .mrc file and what does it contain. Please somebody help me here.

Thanks in advance
Surya

2

This is the definition of the MRC format: CCP-EM: MRC/CCP4 2014 file format

It is a simple data format used to store 2D and 3D image data in medical and biological imaging, especially EM.

In regards your error - you need to provide us with more information about what you are trying to do and what you have done so far. I believe the shapes in the error message are exactly the ones for super-resolution and physical resolution Falcon 3 movies, which is a big hint about where you might be going wrong.

1 Like
3

Thank you for your answer. Regarding the error, I have used the wrong .mrc file, so I got that error. I solved the issue by using the correct .mrc file.

Thank you
Surya