I have picked particles, with CTF estimation and motion correction in WARP. I imported these particles into cryosparc where I did some local CTF refinement and have homogenous refinement/NU refinements from these particles. I am trying to export in order to import into relion to do local classifications. I have encountered an error when copying WARP micrograph coordinates (/star.py:510: FutureWarning: Columnar iteration over characters will be deprecated in future releases.
df[UCSF.IMAGE_ORIGINAL_INDEX], df[UCSF.IMAGE_ORIGINAL_PATH] = ) using cparc2star.py. Althought this still outputs a readable .star file that has the correct coordinates assigned to the particles as checked by eye in a text editor. I can also make a .star file without copying the coordinates from the WARP star file. After imported into relion I get the error ‘box size not available’ after starting a 2D classification job even when --boxsize is specified during csparc2star.py. I am hoping to get into contact with someone who has done this before to see what method you use for this cryosparc to relion conversion.